下面用pfam软件进行结构域的简单分析：
结构域的分析（pfam）
•结构域（structure domain）
qqy 蛋白质三级结构中介于二级和三级结构之间的可以明显区分但又相对独立的折叠单元。
•25~300个氨基酸残基组成。
•五种类型：全平行结构域，反平行结构域，α+β结构域， α/β结构域，其他折叠类型。
蛋白质通常由一个或多个功能区域,通常称为域。不同领域的出现在不同的组合在不同的蛋白质产生不同的曲目在自然界发现的蛋白质。识别领域存在于一种蛋白质可以提供见解,蛋白质的功能。
Pfam软件简介：
有两个等级的品质,pfam家庭:一个和Pfma b。Pfma a条目包含了许多来自底层序列数据库,称为Pfam seq,这是由最近发布的UniProtKB在给定的时间点。Pfam a家庭由许多一个
策划种子含有少量的对齐代表家族成员,剖面隐马尔可夫模型(HMMS)由种子对齐和一个自动生成的全排列,其中包含所有蛋白质序列检测属于家庭定义为HMM搜索数据库的主序列。
Pfam b家庭联合国注释和低质量为他们从集自动生成非冗余的最新加入释放。尽管低的质量,pfam b家庭可以用于识别功能守恒的地区没有发现了pfam a一个条目。
•利用pafm进行蛋白质结构域及功能位点分析
MSRQAWIETSALIECISEYGTKCSFDTFQGLTINDISTLSNLMNQISVASVGFLNDPRTPLQAMSCEFVNFISTADRHAYMLQKNWFDSDVAPNVTTDNFIATYIKPRFSRTVSDVLRQVNNFALQPMENPKLISRQLGVLKAYDIPYSTPINPMDVARSSANVVGNVSQRRALSTPLIQGAQNVTFIVSESDKIIFGTRSLNPIAPGNFQINVPPWYSDLNVVDARIYFTNSFLGCTIQNVQVNAVNGNDPVATITVPTDNNPFIVDSDSVVSLSLSGGAINVTTAVNLTGYAIAIEGKFNMQMNASPSYYTLSSLTIQTSVIDDFGLSAFLEPFRIRLRASGQTEIFSQSMNTLTENLIRQYMPANQAVNIAFVSPWYRFSERARTILTFNQPLLPFASRKLIIRHLWVIMSFIAVFGRYYTVN
窗体底端
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Sequence search results
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Below are the details of the matches that were found. We separate Pfam-A matches into two tables, containing the significant and insignificant matches. A significant match is one where the bits score is greater than or equal to the gathering threshhold for the Pfam domain. Hits which do not start and end at the end points of the matching HMM are highlighted.
The Pfam graphic below shows only the significant matches to your sequence. Clicking on any of the domains in the image will take you to a page of information about that domain. Note that some Pfam-B domains may be obscured by overlapping Pfam-A domains, which are given higher priority when building the graphic.
Pfam does not allow any amino-acid to match more than one Pfam-A family, unless the overlapping families are part of the same clan. In cases where two members of the same clan match the same region of a sequence, only one match is show, that with the lowest E-value.
A small proportion of sequences within the enzymatic Pfam families have had their active sites experimentally determined. Using a strict set of rules, chosen to reduce the rate of false positives, we transfer experimentally determined active site residue data from a sequence within the same Pfam family to your query sequence. These are shown as "Predicted active sites". Full details of Pfam active site prediction process can be found in the accompanying paper.
For Pfam-A hits we show the alignments between your search sequence and the matching HMM. For Pfam-Bs the alignment is between your search sequence and the matching sequence from our library of Pfam-B sequences. You can show individual alignments by clicking on the "Show" button in each row of the result table, or you can show all alignments using the links above each table.
This alignment row for each hit shows the alignment between your sequence and the matching HMM. The alignment fragment includes the following rows:
#HMM:
consensus of the HMM. Capital letters indicate the most conserved positions
#MATCH:
the match between the query sequence and the HMM. A '+' indicates a positive score which can be interpreted as a conservative substitution
#PP:
posterior probability. The degree of confidence in each individual aligned residue. 0 means 0-5%, 1 means 5-15% and so on; 9 means 85-95% and a '*' means 95-100% posterior probability
#SEQ:
query sequence. A '-' indicate deletions in the query sequence with respect to the HMM. Columns are coloured according to the posterior probability
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We found 11 Pfam-A matches to your search sequence (7 significant and 4 insignificant) and 1 Pfam-B match.
Show the search options and sequence that you submitted.
You ran 2 searches, with ID fdf38273-a924-47e0-a3f7-78858e4b47a7
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